How Celiac Disease Is Diagnosed

Diagnosing celiac disease is a fairly lengthy process. In most cases, you'll first have blood tests done and then ultimately have a procedure known as an endoscopy, in which healthcare providers look directly at your small intestine. In the best-case scenario, you'll have your answer within a few days or a week, but this can take much longer in some areas, especially where gastroenterologists are in short supply.

Here's what you can expect as your healthcare provider works to determine if you have celiac disease, plus what you can do to help.

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Self-Checks/At-Home Testing

Some people go onto a gluten-free diet to see if it clears up their symptoms that might be associated with celiac disease. Whether or not this improves your symptoms, it should be followed up with diagnostic testing by your healthcare provider, as your response to the diet is not sufficient for a diagnosis of celiac disease.

Note, however, that going gluten-free can impact test results, so this must be considered when you are being evaluated. Mention your diet changes to your healthcare provider, as your healthcare provider may recommend you return to a regular diet before testing.

You can also consider at-home screening tests for celiac disease, which you can purchase in some stores or online. These use a finger-prick blood sample that you send to a laboratory, and you receive the results in about a week.

If you opt for one of these tests, avoid making changes to your diet in advance, as these results can similarly be affected. A home blood test should always be followed up with medical testing by a healthcare provider.

Medical guidelines recommend celiac disease testing for relatives of those diagnosed with celiac disease since the condition runs in families. Familial risk is due to specific genes.

Some people take advantage of consumer genetic testing, such as through 23andMe, to screen for celiac-related genes. This testing does not require being on a gluten-containing diet. Note, however, that it only shows a risk of the disease, rather than markers of having celiac disease.

Labs and Tests

In most cases, celiac disease blood testing (which can be ordered by your primary care healthcare provider) will be the first step toward a diagnosis. There are several blood tests commonly used to detect celiac disease, although many healthcare providers will only request one or two of them.

These tests look for various antibodies. If your body is undergoing an autoimmune reaction to gluten, one or more of these blood tests should come up positive. This indicates that further testing is needed to see if you truly have celiac disease.

However, it is possible for you to have negative blood test results and still have celiac disease. Some people have a condition known as IgA deficiency that can cause false-negative results on some celiac disease blood tests. If you have this (there's yet another blood test that will look for it), you'll need different tests to screen for celiac disease. In a few other cases, the blood test results simply don't reflect the amount of intestinal damage present.

Therefore, if your blood tests are negative, but your symptoms and family medical history still indicate a strong possibility of celiac disease, you should talk to your healthcare provider about further testing.

Diet and Your Test Results

Because the tests for celiac disease are looking specifically for signs of small intestine damage, you must be eating gluten for the tests to be accurate. If you're not eating gluten-containing foods—or not eating enough of them—it's possible for the testing to come up negative, even if you actually do have celiac disease.

It's ideal to continue eating a normal diet, with gluten-containing foods several times a day, until all your testing is completed.

If you've already started following a gluten-free diet, you may want to consider what's called a gluten challenge, in which you eat a set amount of gluten for some period of time, and then undergo testing for celiac disease. This approach does carry some risks, though, and may not produce the results you want, so talk to your healthcare provider about the potential pros and cons.

Positive Blood Tests With Skin Rash

If you have an itchy, painful blistering skin rash called dermatitis herpetiformis, plus positive celiac blood tests, you're officially diagnosed with celiac disease as well—no further testing required.

That's because studies have shown that nearly everyone with dermatitis herpetiformis—which, like celiac disease, is caused by an autoimmune reaction to gluten—also has intestinal damage from gluten ingestion.

That said, a positive skin biopsy is the gold standard for confirming dermatitis herpetiformis. This looks for deposits of anti-gluten antibodies under your skin. Your healthcare provider may or may not opt for this testing method.

Genetic Testing

In some cases, your healthcare provider may recommend genetic testing for celiac disease. Genetic tests can be done using a swab of your mouth or by drawing blood. As with at-home genetic test kits, what you are currently eating will not affect results of such tests administered in a clinical setting.

Genetic tests can't tell you if you actually have celiac disease—for that, you'll need to undergo the blood tests and endoscopy. If you don't have one of these two main genes, the odds of you having celiac disease are very slim, although some cases of celiac have been documented in people who don't carry either gene.

A positive genetic test for celiac disease doesn't mean you definitely have celiac disease—up to 40% of the population carries one of those genes, and the vast majority never develop celiac. However, it does mean you possibly can develop the condition. You'll need to discuss with your healthcare provider your next steps if your celiac disease gene test comes back positive, especially if you have a family history of celiac disease.


If your celiac disease blood tests come back positive—or if they're negative, but you and your healthcare provider agree on the need for further testing anyway—your next step is a procedure known as an endoscopy. This usually is performed by a gastroenterologist, one of the many types of healthcare providers who treat celiac disease.

In an endoscopy, an instrument with a tiny camera attached is threaded down your throat so your healthcare provider can look directly at the lining of your small intestine to see if villous atrophy is present. In some cases (but not all), the damage from celiac disease can be seen immediately during this procedure.

However, to confirm the diagnosis, the surgeon also will use the instrument to take tiny samples of your intestine. Because damage from celiac disease can be patchy, the surgeon should take at least four to six samples. These samples then will be examined under a microscope by a pathologist (a healthcare provider who diagnoses disease by examining body tissue directly) to determine if your intestines are damaged. If the pathologist sees the damage, you'll be diagnosed with celiac disease.

Some medical centers also are using capsule endoscopy, in which you swallow a pill with a tiny camera to screen for celiac disease. This has the benefit of seeing parts of your small intestine that can't be reached by conventional endoscopy instruments. However, capsule endoscopy doesn't allow the surgeon to take samples of your intestine, and it may not be as accurate in identifying damage as traditional endoscopy.

Just as it's possible to have negative blood tests but a biopsy that shows you have celiac disease, it's also possible to have positive blood tests but a negative biopsy. This is known as latent celiac disease or potential celiac disease.

If you fall into this category, your healthcare provider likely will recommend regular repeat endoscopies, since many people with latent celiac disease ultimately develop full-blown intestinal damage. You also can discuss the possibility of following the gluten-free diet as a trial, to see if any symptoms clear up.

Differential Diagnoses

The combination of positive blood tests and an endoscopy finding of villous atrophy will help rule out other causes of villous atrophy, which include tropical sprue, severe intestinal bacterial overgrowth, eosinophilic enteritis, infectious enteritis, and lymphoma.

Some people who test negative for celiac disease nonetheless still have symptoms that clear up on a gluten-free diet. They may be diagnosed with non-celiac gluten sensitivity, a recently recognized and as-yet poorly-understood condition. This is sometimes called gluten intolerance or gluten allergy (although it is not a true allergy). There are no generally accepted tests to determine if you have gluten sensitivity; the only way to know is to give up gluten and see if your symptoms improve.

A true wheat allergy is another possibility; it's evidenced specific IgE antibodies that are triggered by exposure to wheat allergens and not by the gluten in non-wheat grains. Celiac disease shows an autoimmune process instead, which may exhibit different types of antibodies (IgG and IgA) directed not only against gluten but also against your own cellular components.


If you test negative for celiac disease, that doesn't mean you won't develop it in the future. Close relatives of celiac disease patients (who themselves have a high risk of developing the condition) with a positive celiac antibody blood test may need repeated screening over the course of years to catch all cases.

Therefore, if you have risk factors for celiac disease—"celiac genes," close family members with the condition, other autoimmune diseases, or even just related symptoms—you may want to consider setting up a regular testing schedule with your healthcare provider. If you are diagnosed with celiac disease early, you can minimize the damage to your body and potentially ward off complications, such as osteoporosis and malnutrition.

A Word From Verywell

Positive test results for celiac disease on your endoscopy and biopsy mean you definitely have the condition and must follow the gluten-free diet for life. Although this can seem overwhelming at first, you ultimately may find your health improves dramatically and that eating gluten-free becomes just another part of your way of life. Truthfully, there are many, many foods you can eat when you're gluten-free.

Frequently Asked Questions

  • What is the most reliable blood test for celiac disease?

    Among the various tests that can help to home in on a diagnosis of celiac disease, the one regarded as the gold standard is the tTG-IgA test. It looks for antibodies called tissue transglutaminase IgA in people who are eating gluten. For children 2 and under, testing for deamidated gliadin IgA should be included. The tTg-IgA test is positive in 98% of people who have celiac disease.

  • What procedure is used to definitively diagnose celiac disease?

    The only way to tell for sure if a person has celiac disease is with an intestinal, or duodenal, biopsy. It involves threading a long, thin scope with a camera at the end down the throat to the small intestine. This provides a view of the cilia lining the small intestine and makes it possible to extract a small sample of tissue for examination in a lab. It usually is done on an outpatient basis.

  • What lab findings indicate celiac disease?

    Pathologists who study duodenal samples for celiac disease use a system called Marsh classification to determine if a lab sample has histological changes that indicate celiac disease. The system is based on certain characteristics of the tissue to assign it a type between 0 and 4. If you have an intestinal biopsy that comes back as a type 3, you will be diagnosed with celiac disease.

  • What kind of healthcare provider can diagnose celiac disease?

    Typically, a gastroenterologist will diagnose celiac disease. These healthcare providers specialize in conditions that affect all organs of the digestive tract, including the small intestine, and are trained to do endoscopies.

  • Is there a screening test for celiac disease?

    There is no routine screening for celiac disease, but certain people who potentially are at risk should be tested, according to the Celiac Disease Foundation:

    • Children over 3 and adults with symptoms of celiac disease
    • First-degree relatives of people who have celiac disease
    • People with certain autoimmune disorders and other conditions that are strongly associated with celiac disease—type 1 diabetes, autoimmune liver disease, Down syndrome, Turner syndrome, Williams syndrome, and selective immunoglobulin A (IgA) deficiency
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Verywell Health uses only high-quality sources, including peer-reviewed studies, to support the facts within our articles. Read our editorial process to learn more about how we fact-check and keep our content accurate, reliable, and trustworthy.
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Additional Reading

By Jane Anderson
Jane Anderson is a medical journalist and an expert in celiac disease, gluten sensitivity, and the gluten-free diet.