Does Splenda Cause Cancer?

Does the artificial sweetener Splenda (sucralose) cause cancer? Is it a safe substitute for sugar in a cancer-prevention diet?

If you google the question "Does Splenda cause cancer," you'll get answers saying both yes and no. Some articles quote studies that found leukemia in mice and say yes. In contrast, you may read that Splenda does not increase cancer risk and is considered safe, even in pregnant and breastfeeding women. Which answer is correct?

Packages of Splenda and Equal
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What Is Splenda (Sucralose)?

Splenda, known by the generic name sucralose, is a non-nutritive sweetener that is 600 times sweeter than table sugar. It was first approved in 1998 as a table-top sugar substitute, and in 1999 it was approved as a general-purpose sweetener. Splenda is now estimated to be present in more than 4,000 products, from dessert mixes to syrup, and is available in more than 80 countries.

Sucralose is made by starting with regular white table sugar (sucrose). The difference is that, in the artificial sweetener, three hydrogen-oxygen bonds are replaced with three chlorine atoms. Sucralose interacts with nerve cells (chemoreceptors) in the digestive tract that play a role in our brain's interpreting a sweet taste sensation.

If you are confused by the different sugar substitutes currently available, here is a comparison of artificial sweeteners that are currently used in the United States.

The Controversy Over Artificial Sweeteners

There has been controversy surrounding sugar substitutes. The cancer stigma that surrounds artificial sweeteners is believed to stem from the 1970s when lab rats developed bladder cancer during a saccharin trial. Although no case of cancer in a human has been linked to saccharin, the stigma remains and has continued with the approval of aspartame (sold as Equal or NutraSweet), which is likely of more concern than Splenda.

On the other side of the equation is the large amount of sugar the average American consumes—reportedly 22 teaspoons daily—combined with rising rates of obesity and diabetes.

Below, we'll look at Splenda alone and what we have learned about whether it may cause cancer or lead to health problems that increase cancer risk.

Understanding Carcinogenicity/Genotoxicity

Before discussing the studies, it's helpful to define a few terms. Carcinogenicity refers to the ability of a substance to cause cancer. Genotoxicity refers to the ability of a substance to damage genes (genes are located within DNA, which is in the nucleus of every cell).

Cancer usually begins when a series of gene mutations and other genetic damage cause a cell to divide and grow out of control. In other words, it is genotoxicity (the ability to damage genes) that usually makes a substance carcinogenic.

The Regulatory Answer to Whether Sucralose Causes Cancer

It's important to begin with the regulatory committee's decision on whether Splenda can cause cancer. Based on more than 110 studies (physiochemical and pharmacokinetic/toxicokinetic studies) conducted in the lab, in animals, and in humans, the FDA has approved the use of sucralose in the consumer market without restrictions.

In addition, studies evaluating metabolites—the products of sucralose as it is broken down and metabolized by the body—were also found to have no carcinogenic potential. Overall, sucralose has been found to have no potential for carcinogenicity or genotoxicity, even at high doses, in both in vitro and in vivo studies. In vitro studies are performed in the lab, usually in a dish, while in vivo studies evaluate the way a substance interacts in the body of either laboratory animals or humans.

The Studies

We've heard what the FDA has to say, but let's talk about what the studies say, what they don't say, and what hasn't been studied so that you can make your own educated decision about whether you wish to include Splenda in your diet.

Most studies have not shown any increase in cancer risk with Splenda, with the exception of a 2016 Italian study. In this study, which looked at the effect of sucralose in Swiss mice, it was found that the male mice exposed to high doses of sucralose had an increased risk of developing leukemia. A follow-up study by the manufacturer failed to show this association. But what did the study actually test?

The sucralose and leukemia study looked at mice who were given sucralose in three different doses beginning in utero (prenatally) and throughout their lifespan. At doses equivalent to ordinary human doses, there was no increased risk of leukemia. There was, however, an association at doses roughly equivalent to four times the recommended daily intake in humans when used throughout the mouse lifespan.

A study such as this is difficult to interpret. Certainly, most adults are not going to use four times the recommended maximum amount of sucralose every day of their lives. But what amount is safe? In general, it's thought that there is no safe limit to a carcinogen. This is also only one study conducted in animals—though it was relatively large compared to other studies.

Compared to many risk factors in our lives, if this does mean an increased cancer risk, it is probably small relative to other risk factors we are exposed to daily. For example, it's thought that home exposure to radon causes 21,000 lung cancer deaths annually, but many people don't take the time to purchase a 10-dollar test kit to find out if their home has a problem.

Sucrose (Sucralose) and Inflammatory Bowel Disease

Sucralose has also been found to have a few actions of concern in the digestive tract. In other words, it is not "inert" or completely inactive. Since this article is addressing possible cancer risk, we will stick with the findings that could possibly have implications for the formation of cancer—even if distant.

Splenda (sucralose) appears to decrease the number of "good" bacteria in the gut. We are learning that having enough good bacteria in the gut is as important or more important than having "bad" bacteria in the gut. It's not certain if this has any significance, or if this is related to another finding—that sucralose is a risk factor for inflammatory bowel disease.

We need to be clear, however, that saying something is a risk factor does not mean it is a cause. For example, older age is a risk factor for many cancers but is not a cause of cancer. Inflammatory bowel disease (IBD) includes conditions such as Crohn's disease and ulcerative colitis.

We know that inflammatory bowel disease raises colon cancer risk. In addition, some treatments for IBD elevate cancer risk. Knowing this, we can't necessarily jump to the conclusion that sucralose could cause cancer (by predisposing to IBD, which in turn predisposes someone to cancer), but it's still important to be asking this question.

Splenda May Increase Appetite and Affect Obesity

You're probably familiar with a study that rocked the airwaves: soda containing artificial sweeteners may actually increase the risk of obesity. Many studies have looked at artificial sweeteners for weight loss, but Splenda (sucralose) has been found to increase appetite in at least one study. Since obesity is a strong risk factor for cancer (almost equal to the risk associated with smoking), and diabetes (often related to obesity) is also an independent risk factor for cancer, this is an important topic.

The finding that sucralose may increase appetite is fairly ironic given that the compound is often used to avoid the calories associated with sugar. An equally serious concern, however, is that the average American consumes far too much sugar, while obesity has become nearly epidemic.

Splenda (Sucralose) and Heat

Safety studies have been done looking at the effects and stability of sucralose under normal conditions of use. Some researchers have examined, however, what happens when sucralose is exposed to heat, such as with cooking. In this setting, (even with mild heating) there is a bit more concern. Cooking sucralose at high temperatures generates compounds known as chloropropanols, which are potentially toxic compounds. If you wish to avoid this potential risk, do not cook or bake with Splenda.

Ecological Impact of Splenda

Since sucralose gets into the water supply and is present in groundwater, scientists have been trying to study what—if any—effect this may have ecologically. At this time, we simply aren't sure.

Bottom Line

At the present time, there is little evidence that sucralose—used in normal amounts and not heated—contributes to cancer risk. Following the "everything in moderation" rule, a little Splenda is probably not worth fretting over for those who crave sweetener.

It's important to note that while many people are concerned about what we still don't know about artificial sweeteners, there are probably many other risks in our lives that may be more deserving of our focus.

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9 Sources
Verywell Health uses only high-quality sources, including peer-reviewed studies, to support the facts within our articles. Read our editorial process to learn more about how we fact-check and keep our content accurate, reliable, and trustworthy.
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