How Do I Get Tested For Hepatitis B/HBV?

There are three blood tests for Hepatitis B. One tests for the virus itself, and two test for your body’s reaction to the virus. Sometimes your healthcare provider will do all three tests because each can supply different information.

Hepatitis B virus test
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  • If you are positive for the antigen test (HBsAg) it means that you are currently infected with the virus and can pass it to other. If you clear an HBV infection and do not remain chronically infected, you will usually test negative again within 4 months after your symptoms resolve.
  • If you are positive on the anti-HBs test, which looks for your body’s reaction to a surface protein of the virus, it means that you are immune to Hepatitis B. This could either be because you were previously exposed to the virus or because you were vaccinated. You can not pass the virus to others.
  • If you are positive on the anti-HBc test, which looks for your body’s reaction to a core protein of the virus, it usually means that you are chronically infected with HBV, and can pass the disease to others. However, if you are also positive on the anti-HBs test, then a positive anti-HBc test is probably because of a previous infection.

Other tests may be ordered if your healthcare provider thinks you are chronically infected with HBV. These tests are done to monitor the progress of the disease and its treatment—not to detect whether or not you are infected.

There is a vaccine for hepatitis B. Current guidelines recommend that all infants and children be vaccinated against HBV, in addition to adults aged 19-59 who have not previously been vaccinated.  

Unvaccinated adults aged 60+ who are at a high risk of contracting the virus should talk to their healthcare provider about getting vaccinated. Risk factors include multiple sex partners, a sexual partner with HBV, living with someone with chronic HBV, a job that brings you in contact with human blood, injection drug use, and being a man who has sex with men. Anyone 60 and older may receive hepatitis B vaccination if they choose to, even if they don’t meet risk-based recommendations.

1 Source
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  1. Murthy N, Wodi AP, Bernstein H, McNally V, Cineas S, Ault K. Advisory Committee on Immunization Practices Recommended Immunization Schedule for Adults Aged 19 Years or Older — United States, 2022. MMWR Morb Mortal Wkly Rep. 2022;71:229–233. doi:10.15585/mmwr.mm7107a1.

Additional Reading
  • CDC. (2015) Hepatitis B In Short. Accessed 11/11/2015 at
  • Merrill RM, Hunter BD. Seroprevalence of markers for hepatitis B viral infection. Int J Infect Dis. 2011 Feb;15(2):e78-121. doi: 10.1016/j.ijid.2010.09.005.
  • National Clinical Guideline Centre (UK). Hepatitis B (Chronic): Diagnosis and Management of Chronic Hepatitis B in Children, Young People and Adults. London: National Institute for Health and Care Excellence (UK); 2013 Jun.
  • Tillmann HL. Hepatitis C virus core antigen testing: role in diagnosis, disease monitoring and treatment. World J Gastroenterol. 2014 Jun 14;20(22):6701-6. doi: 10.3748/wjg.v20.i22.6701.

By Elizabeth Boskey, PhD
Elizabeth Boskey, PhD, MPH, CHES, is a social worker, adjunct lecturer, and expert writer in the field of sexually transmitted diseases.